4AMJ
Turkey beta1 adrenergic receptor with stabilising mutations and bound biased agonist carvedilol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.056, 62.196, 100.926 |
| Unit cell angles | 90.00, 109.17, 90.00 |
Refinement procedure
| Resolution | 95.330 - 2.300 |
| R-factor | 0.20354 |
| Rwork | 0.202 |
| R-free | 0.24005 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y02 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.394 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.100 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.590 |
| Number of reflections | 47122 | |
| <I/σ(I)> | 11.3 | 2.1 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 6.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 277 | 0.1 M BICINE PH 9.0, 22% PEG 600, 4 DEGREES CELSIUS. |
