4AM8
Crystal structure of the R54G mutant of putrescine transcarbamylase from Enterococcus faecalis bound to a curing guanidinium ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 103.690, 130.600, 164.157 |
| Unit cell angles | 90.00, 104.25, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.990 |
| R-factor | 0.18335 |
| Rwork | 0.182 |
| R-free | 0.20571 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a8p |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.108 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 30.000 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 130385 | |
| <I/σ(I)> | 8.6 | 2 |
| Completeness [%] | 90.0 | 82 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.02 M MAGNESIUM CHLORIDE, 30 % PEG 550 MONOMETHYLETHER, 0.1 M HEPES PH 7.5, 0.001 M PALO, 0.05 M GUANIDINIUM CHLORIDE |
