4AKB
Structure of Galactose Binding lectin from Champedak (CGB) with Galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.286, 121.030, 77.638 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.550 - 1.950 |
| R-factor | 0.1725 |
| Rwork | 0.171 |
| R-free | 0.19740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ak4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.610 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.110 | 0.800 |
| Number of reflections | 52244 | |
| <I/σ(I)> | 17.7 | 2.1 |
| Completeness [%] | 98.5 | 96 |
| Redundancy | 10.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 40% PEG 600, 100 MM PHOSPHATE/CITRATE BUFFER PH 4.2 |
