4AJY
von Hippel-Lindau protein-ElonginB-ElonginC complex, bound to Hif1- alpha peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-22 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.232, 59.232, 244.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.500 - 1.730 |
| R-factor | 0.19241 |
| Rwork | 0.190 |
| R-free | 0.23241 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lm8 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.178 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.840 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.060 | 0.540 |
| Number of reflections | 46307 | |
| <I/σ(I)> | 22.19 | 3.09 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 7.4 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M K PHOSPHATE PH 6.6 0.2 M (NH4)2SO4 20% PEG MME 5000 5 MM DTT |
