4AFL
The crystal structure of the ING4 dimerization domain reveals the functional organization of the ING family of chromatin binding proteins.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 128.450, 186.600, 62.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.688 - 2.275 |
| R-factor | 0.2073 |
| Rwork | 0.205 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.995 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.090 | 2.400 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.090 | 0.360 |
| Number of reflections | 34302 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 96.8 | 96.8 |
| Redundancy | 6.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 24% PEG 3350, 0.1 M BISTRIS- PROPANE PH:7.5, 0.4 M SODIUM NITRATE, 10% OPTISALT SOLUTION 6, SILVER BULLET ADDITIVE 3. |
