4ADF

CRYSTAL STRUCTURE OF THE HUMAN COLONY-STIMULATING FACTOR 1 (hCSF-1) CYTOKINE IN COMPLEX WITH THE VIRAL RECEPTOR BARF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-11
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 31 2 1
Unit cell lengths	218.440, 218.440, 331.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	75.847 - 4.400
R-factor	0.2395
Rwork	0.238
R-free	0.27630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2CH8 3uf2
RMSD bond length	0.006
RMSD bond angle	1.292
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.850	4.510
High resolution limit [Å]	4.400	4.400
Rmerge	0.120	0.810
Number of reflections	58151
<I/σ(I)>	13.5	2.8
Completeness [%]	99.4	98.2
Redundancy	5.6	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.1 M TRIS HCL, PH 8.50, 1.0 M AMMONIUM PHOSPHATE MONOBASIC