4ADF

CRYSTAL STRUCTURE OF THE HUMAN COLONY-STIMULATING FACTOR 1 (hCSF-1) CYTOKINE IN COMPLEX WITH THE VIRAL RECEPTOR BARF1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	SECRETED PROTEIN BARF1	polymer	208	23412.7	12	UniProt (P0CW72) Pfam (PF22305) UniProt (by SIFTS) (P03228) In PDB	HUMAN HERPESVIRUS 4	BARF1,33 KDA EARLY PROTEIN, P33
2	G, H, I, J, K...	MACROPHAGE COLONY-STIMULATING FACTOR 1	polymer	153	17868.2	12	UniProt (P09603) Pfam (PF05337) In PDB	HOMO SAPIENS (HUMAN)	HCSF-1, CSF-1, M-CSF, MCSF, LANIMOSTIM, PROCESSED MACROPHAGE COLONY-STIMULATING FACTOR 1
3	e, f, h, Y	alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	4	In PDB GlyTouCan (G34442SS)
4	a, i, j, Z	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	4	In PDB GlyTouCan (G55220VL)
5	b, c, d, g	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	4	In PDB GlyTouCan (G22768VO)

Sequence modifications

A, B, C, D, E, F, M, N, O, P, Q, R: 21 - 221 (UniProt: P0CW72)

PDB	External Database	Details
Lys 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
Ser 169	Thr 169	engineered mutation

G, H, I, J, K, L, S, T, U, V, W, X: 1 - 149 (UniProt: P09603)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	495370.5
Branched	Number of molecules	12
	Total formula weight	12875.6
All*	Total formula weight	508246.0

*Water molecules are not included.