4AA7
E.coli GlmU in complex with an antibacterial inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2005-10-18 |
| Detector | RIGAKU CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 80.713, 80.713, 140.061 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.360 - 2.000 |
| R-factor | 0.1794 |
| Rwork | 0.177 |
| R-free | 0.22128 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3twd |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.992 |
| Data reduction software | d*TREK |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.360 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.460 |
| Number of reflections | 33180 | |
| <I/σ(I)> | 9 | 2.5 |
| Completeness [%] | 95.0 | 82 |
| Redundancy | 3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 19-22% (W/V) PEG3350, 100MM PCTP PH 5.5 AND 400MM AMMONIUM SULFATE, VAPOR DIFFUSION, HANGING DROP |
