4A7W

Crystal structure of uridylate kinase from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-21
Detector	ADSC CCD
Spacegroup name	H 3 2
Unit cell lengths	137.420, 137.420, 152.891
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.288 - 1.800
R-factor	0.1903
Rwork	0.188
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bnd
RMSD bond length	0.006
RMSD bond angle	1.038
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	0.500
Number of reflections	51462
<I/σ(I)>	37.3	4.3
Completeness [%]	99.9	100
Redundancy	11.1	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1