4A50
Crystal structure of human kinesin Eg5 in complex with 2-Amino-5-(3-methylphenyl)-5,5-diphenylpentanoic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-11-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 158.540, 158.540, 158.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.030 - 2.750 |
| R-factor | 0.19989 |
| Rwork | 0.198 |
| R-free | 0.24301 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ken |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.747 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.080 | 0.610 |
| Number of reflections | 17322 | |
| <I/σ(I)> | 12.2 | 3.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 23 % POLYETHYLENE GLYCOL-3350, 0.25 M AMMONIUM SULPHATE AND 0.1 M MES PH 5.5 |
