4A4C
Structure of phosphoTyr371-c-Cbl-UbcH5B-ZAP-70 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 75.285, 75.285, 459.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.325 - 2.704 |
| R-factor | 0.2059 |
| Rwork | 0.203 |
| R-free | 0.26770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2ESK 1fbv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.308 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.060 | 0.640 |
| Number of reflections | 22290 | |
| <I/σ(I)> | 29.9 | 4.2 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 16.8 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | 50 MM HEPES, PH 7.5, 0.2 M KCL AND 31-35% (V/V) PENTAERYTHRITOL PROPOXYLATE (5/4 PO/OH) AT 4 DEGREES CELSIUS. |
