4A49
Structure of phosphoTyr371-c-Cbl-UbcH5B complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-19 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 115.877, 115.877, 52.572 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.297 - 2.214 |
R-factor | 0.1699 |
Rwork | 0.168 |
R-free | 0.20550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2ESK AND 1FBV |
RMSD bond length | 0.008 |
RMSD bond angle | 1.185 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.270 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.080 | 0.640 |
Number of reflections | 20424 | |
<I/σ(I)> | 11.6 | 2.8 |
Completeness [%] | 99.6 | 100 |
Redundancy | 6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 291 | 20% (W/V) PEG 3350 AND 0.2 M POTASSIUM THIOCYANATE AT 18 DEGREES CELSIUS. |