4A36
Structure of duck RIG-I helicase domain bound to 19-mer dsRNA and ATP transition state analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-05 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 |
| Unit cell lengths | 129.270, 129.270, 106.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.950 - 3.700 |
| R-factor | 0.19628 |
| Rwork | 0.193 |
| R-free | 0.26270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a2p |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.190 | 0.970 |
| Number of reflections | 18825 | |
| <I/σ(I)> | 10.8 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.55 | 7.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12 MG/ML, 1.2 TIMES MOLAR RATIO OF 19-MER DSRNA, 4 MM ADP, 2 MM ALF3 AND 0.1 M HEPES PH 7.5, 5% (V/V) ISO-PROPANOL AND 10% (W/V) PEG 4000. |
