4A21
Structure of Mycobacterium tuberculosis fructose 1,6-bisphosphate aldolase bound to sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 336.945, 43.234, 103.020 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 42.873 - 2.350 |
| R-factor | 0.2151 |
| Rwork | 0.211 |
| R-free | 0.26100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.984 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.500 |
| High resolution limit [Å] | 2.350 |
| Rmerge | 0.110 |
| Number of reflections | 71704 |
| <I/σ(I)> | 3.8 |
| Completeness [%] | 99.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 8000, 1.8 M LI2SO4, 50 MM TRIS/HOAC PH 7.5. |
