4A0C
Structure of the CAND1-CUL4B-RBX1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.090, 152.360, 263.010 |
| Unit cell angles | 90.00, 89.37, 90.00 |
Refinement procedure
| Resolution | 47.760 - 3.800 |
| R-factor | 0.24206 |
| Rwork | 0.238 |
| R-free | 0.31856 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1U6G 2hye |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.139 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.900 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.090 | 0.510 |
| Number of reflections | 59850 | |
| <I/σ(I)> | 10.2 | 2.8 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 100 MM MES PH 6.3, 30% PEG 200, 2% PEG 8000. |
