43C9

CRYSTALLOGRAPHIC STRUCTURE OF THE ESTEROLYTIC AND AMIDOLYTIC 43C9 ANTIBODY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1997-05
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	104.130, 112.660, 245.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.21 *
Rwork	0.210
R-free	0.26500
Structure solution method	OTHER
RMSD bond length	0.011
RMSD bond angle	1.752
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.081 *
Total number of observations	234292 *
Number of reflections	65372
<I/σ(I)>	16	2
Completeness [%]	89.7	0.564
Redundancy	3.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5		80% NACL, 50 MM MOPS PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		70-80 (%)
2	1	reservoir	MOPS	50 (mM)