421D

5'-D(*TP*TP*CP*TP*TP*(BRO)CP*TP*TP*C)-3', 5'-R(*GP*AP*AP*GP*AP*AP*GP*AP*A)-3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	263
Detector technology	IMAGE PLATE
Collection date	1997-02-15
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 61
Unit cell lengths	48.080, 48.080, 42.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.201
Rwork	0.201
R-free	0.23700
RMSD bond length	0.008
RMSD bond angle	7.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.040	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.069	0.355
Number of reflections	4948
Completeness [%]	89.3	77.2
Redundancy	3.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	hybrid	1 (mM)	double strand
2	1	drop	cobalt hexamine	0.5 (mM)
3	1	drop	spermine tetrachloride	5 (mM)
4	1	drop	sodium cacodylate	80 (mM)	pH7.0
5	1	drop	MPD	10 (%(v/v))
6	1	reservoir	MPD	40 (%)