3ZYG
NETRING2 LAM AND EGF1 DOMAINS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Wavelength(s) | 1.0, 0.979400 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.500, 64.880, 120.080 |
| Unit cell angles | 90.00, 103.06, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.25056 |
| Rwork | 0.248 |
| R-free | 0.29684 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tvg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.862 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 2.400 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.320 | 0.840 |
| Number of reflections | 42208 | |
| <I/σ(I)> | 6.4 | 2.35 |
| Completeness [%] | 98.9 | 96.6 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% MME550, 0.1M NACL, 0.1M BICINE PH9 |
