3ZY5
Crystal structure of POFUT1 in complex with GDP-fucose (crystal-form-I)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 90 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.543, 42.530, 97.663 |
Unit cell angles | 90.00, 107.63, 90.00 |
Refinement procedure
Resolution | 93.080 - 1.960 |
R-factor | 0.21915 |
Rwork | 0.218 |
R-free | 0.26752 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zy2 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.432 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.010 | 2.060 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.110 | 0.480 |
Number of reflections | 42027 | |
<I/σ(I)> | 12.19 | 12.19 |
Completeness [%] | 98.0 | 94.7 |
Redundancy | 7.06 | 5.01 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |