3ZY3
Crystal structure of POFUT1 in complex with GDP (crystal-form-III)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 65 |
| Unit cell lengths | 132.180, 132.180, 77.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 114.470 - 1.860 |
| R-factor | 0.20546 |
| Rwork | 0.205 |
| R-free | 0.24457 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zy2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.364 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.980 | 1.960 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 64057 | |
| <I/σ(I)> | 22.7 | 3.1 |
| Completeness [%] | 98.8 | 97.4 |
| Redundancy | 9.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
