3ZVY
PHD finger of human UHRF1 in complex with unmodified histone H3 N- terminal tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 110 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 42.300, 42.300, 182.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.210 - 1.950 |
R-factor | 0.1836 |
Rwork | 0.181 |
R-free | 0.22700 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.007 |
RMSD bond angle | 1.107 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.400 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.050 | 0.530 |
Number of reflections | 22160 | |
<I/σ(I)> | 21.46 | 3.12 |
Completeness [%] | 95.8 | 83.9 |
Redundancy | 4.96 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 290 | CRYSTALS WERE OBTAINED AT 17 DEGREES USING THE VAPOR DIFFUSION METHOD BY MIXING A PROTEIN SOLUTION AT A CONCENTRATION OF 606 MICROM (IN 20 MM TRIS PH 7, 150 MM NACL, 0.5 MM TCEP, 25 MICROM ZNCL2 AND 0.1 MM PMSF) AND 10-FOLD EXCESS OF PEPTIDE WITH AN EQUAL VOLUME OF RESERVOIR SOLUTION (0.1 M TRIS PH 8.5, 0.2 M MGCL2, 30 % PEG 4000). CRYSTALS WERE CRYOPROTECTED WITH 15 % MPD AND FLASH FROZEN IN LIQUID NITROGEN. |