3ZVN
The structural basis for substrate recognition by mammalian polynucleotide kinase 3' phosphatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-14 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.270, 72.990, 114.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.495 - 2.150 |
R-factor | 0.2082 |
Rwork | 0.205 |
R-free | 0.26890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yj5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.298 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.500 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.110 | 0.660 |
Number of reflections | 28753 | |
<I/σ(I)> | 8.5 | 2.3 |
Completeness [%] | 89.2 | 92.7 |
Redundancy | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M BIS TRIS PH 5.5, 0.1 M AMMONIUM ACETATE, 17 % (W/V) PEG 10000 |