3ZTP
Orthorhombic crystal form P21212 of the Aquifex aeolicus nucleoside diphosphate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 52.180, 95.120, 61.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.313 - 1.370 |
| R-factor | 0.1454 |
| Rwork | 0.144 |
| R-free | 0.16160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zto |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.325 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.310 | 1.430 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.060 | 0.310 |
| Number of reflections | 65505 | |
| <I/σ(I)> | 12.4 | 2.5 |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 3.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2.0 M AMMONIUM FORMATE, 0.1 M HEPES, PH 7.5 |
