3ZTN

STRUCTURE OF INFLUENZA A NEUTRALIZING ANTIBODY SELECTED FROM CULTURES OF SINGLE HUMAN PLASMA CELLS IN COMPLEX WITH HUMAN H1 INFLUENZA HAEMAGGLUTININ.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 41 3 2
Unit cell lengths	206.100, 206.100, 206.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.162 - 3.001
R-factor	0.218
Rwork	0.214
R-free	0.28910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1RD8 3f12 1za6
RMSD bond length	0.009
RMSD bond angle	1.277
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.160	3.160
High resolution limit [Å]	3.000	3.000
Rmerge	0.050	0.300
Number of reflections	30516
<I/σ(I)>	11.8	2.8
Completeness [%]	100.0	100
Redundancy	8.4	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1