3ZTJ
Structure of influenza A neutralizing antibody selected from cultures of single human plasma cells in complex with human H3 Influenza haemagglutinin.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 77 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 171.488, 193.428, 213.748 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 143.420 - 3.410 |
R-factor | 0.23671 |
Rwork | 0.234 |
R-free | 0.28434 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.699 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 143.400 | 3.550 |
High resolution limit [Å] | 3.400 | 3.410 |
Rmerge | 0.120 | 0.600 |
Number of reflections | 97028 | |
<I/σ(I)> | 14 | 1.6 |
Completeness [%] | 96.1 | 93.2 |
Redundancy | 2.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |