3ZPR
Thermostabilised turkey beta1 adrenergic receptor with 4-methyl-2-(piperazin-1-yl) quinoline bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.968, 60.812, 101.178 |
| Unit cell angles | 90.00, 109.20, 90.00 |
Refinement procedure
| Resolution | 95.550 - 2.700 |
| R-factor | 0.22594 |
| Rwork | 0.224 |
| R-free | 0.26621 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4amj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.428 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.300 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.150 | 0.420 |
| Number of reflections | 27544 | |
| <I/σ(I)> | 13.4 | 1.9 |
| Completeness [%] | 96.1 | 91.2 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 277 | 0.1M BICINE PH9.0, 24% PEG600, 4C |
