3ZPO
Crystal structure of JmjC domain of human histone demethylase UTY with bound GSK J1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-16 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.158, 110.755, 119.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.510 - 2.000 |
| R-factor | 0.17906 |
| Rwork | 0.177 |
| R-free | 0.20977 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zli |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.395 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.830 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.770 |
| Number of reflections | 81514 | |
| <I/σ(I)> | 10.4 | 2 |
| Completeness [%] | 99.1 | 97.1 |
| Redundancy | 6.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15%(W/V) PEG 3350, 0.1M MAGNESIUM FORMATE |
