3ZOJ
High-resolution structure of Pichia Pastoris aquaporin Aqy1 at 0.88 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-16 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | I 4 |
Unit cell lengths | 90.763, 90.763, 80.311 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.150 - 0.880 |
R-factor | 0.10329 |
Rwork | 0.103 |
R-free | 0.10739 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w2e |
RMSD bond length | 0.018 |
RMSD bond angle | 1.691 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.000 | 0.930 |
High resolution limit [Å] | 0.880 | 0.880 |
Rmerge | 0.060 | 0.870 |
Number of reflections | 252057 | |
<I/σ(I)> | 26.2 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.8 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 26% PEG600, 100 MM TRIS (PH=8.0), 200 MM CACL2 |