3ZOJ
High-resolution structure of Pichia Pastoris aquaporin Aqy1 at 0.88 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | I 4 |
| Unit cell lengths | 90.763, 90.763, 80.311 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.150 - 0.880 |
| R-factor | 0.10329 |
| Rwork | 0.103 |
| R-free | 0.10739 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w2e |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.000 | 0.930 |
| High resolution limit [Å] | 0.880 | 0.880 |
| Rmerge | 0.060 | 0.870 |
| Number of reflections | 252057 | |
| <I/σ(I)> | 26.2 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.8 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 26% PEG600, 100 MM TRIS (PH=8.0), 200 MM CACL2 |
