3ZNV
Crystal structure of the OTU domain of OTULIN at 1.3 Angstroms.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2011-11-19 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.490, 72.010, 94.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.280 - 1.300 |
| R-factor | 0.1268 |
| Rwork | 0.125 |
| R-free | 0.15439 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.663 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.260 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.110 | 0.330 |
| Number of reflections | 73748 | |
| <I/σ(I)> | 7.3 | 3.3 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM MES/IMIDAZOLE PH 6.5, 30 MM MGCL2, 30 MM CACL2, 10% PEG 4000, 20% GLYCEROL |
