3ZNI
Structure of phosphoTyr363-Cbl-b - UbcH5B-Ub - ZAP-70 peptide complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 94.970, 131.800, 122.000 |
| 格子定数 [度] | 90.00, 91.92, 90.00 |
精密化法
| 残基 | 30.540 - 2.210 |
| R因子 | 0.1767 |
| Rwork | 0.175 |
| R-free | 0.21140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3PFV 2ldr 4A49 AND 4AUQ |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.004 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.811 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.500 | 2.270 |
| 分解能 [Å] (高) | 2.210 | 2.210 |
| Rmerge_l_obs | 0.060 | 0.690 |
| 独立反射数 | 149230 | |
| <I/σ(I)> | 14.2 | 2.1 |
| 完全性 [%] | 99.6 | 99.9 |
| 冗長性 | 3.4 | 3.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 0.1 M BICINE, PH 9.0, 8-11% (W/V) PEG 3350 AND 0.1 M SODIUM FORMATE | |||
| 1 | 0.1 M BICINE, PH 9.0, 8-11% (W/V) PEG 3350 AND 0.1 M SODIUM FORMATE |
