3ZN0
LSD1-CoREST in complex with PRSFAA peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.910, 180.380, 234.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.530 - 2.800 |
| R-factor | 0.22906 |
| Rwork | 0.229 |
| R-free | 0.24415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y48 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.917 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.580 |
| Number of reflections | 62542 | |
| <I/σ(I)> | 9.3 | 1.1 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
