3ZMZ
LSD1-CoREST in complex with PRSFAV peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE PX |
| Synchrotron site | SLS |
| Beamline | PX |
| Temperature [K] | 100 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 118.950, 179.270, 234.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.450 - 3.000 |
| R-factor | 0.20848 |
| Rwork | 0.208 |
| R-free | 0.22705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y48 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.902 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 0.540 |
| Number of reflections | 50342 | |
| <I/σ(I)> | 10.6 | 1.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
