3ZML
Anopheles funestus glutathione-s-transferase epsilon 2 (GSTe2) protein structure from different alelles: A single amino acid change confers high level of DDT resistance and cross resistance to permethrin in a major malaria vector in Africa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-02 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.250, 73.040, 129.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.484 - 1.639 |
R-factor | 0.1891 |
Rwork | 0.188 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2imi |
RMSD bond length | 0.021 |
RMSD bond angle | 1.941 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.480 | 1.730 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.130 | 0.700 |
Number of reflections | 58184 | |
<I/σ(I)> | 9 | 3.4 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 6.1 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 25% W/V PEG 1500 AND 0.1M PCB, BUFFER PH 6.0 |