3ZKJ
Crystal Structure of Ankyrin Repeat and Socs Box-Containing Protein 9 (Asb9) in Complex with Elonginb and Elonginc
Replaces: 2XAIExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Collection date | 2010-02-27 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 102.860, 110.740, 113.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.470 - 2.580 |
R-factor | 0.2289 |
Rwork | 0.227 |
R-free | 0.26507 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.520 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.470 | 2.720 |
High resolution limit [Å] | 2.580 | 2.580 |
Rmerge | 0.100 | 0.770 |
Number of reflections | 41297 | |
<I/σ(I)> | 10.7 | 2.1 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.7 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2M NA2SO4, 0.1M BTPROP 6.5, 20% PEG 3350, 10% ETGLY |