3ZKF
Structure of LC8 in complex with Nek9 phosphopeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 63 |
Unit cell lengths | 154.868, 154.868, 47.729 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.967 - 2.600 |
R-factor | 0.2222 |
Rwork | 0.220 |
R-free | 0.26860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.196 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.730 | 2.630 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.100 | 1.000 |
Number of reflections | 22982 | |
<I/σ(I)> | 7.6 | 1.5 |
Completeness [%] | 99.2 | 95.6 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |