3ZJD
A20 OTU domain in reduced, active state at 1.87 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.690, 68.970, 84.300 |
| Unit cell angles | 99.04, 100.09, 96.95 |
Refinement procedure
| Resolution | 34.292 - 1.870 |
| R-factor | 0.1835 |
| Rwork | 0.182 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vfj |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.755 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.840 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.050 | 0.450 |
| Number of reflections | 73826 | |
| <I/σ(I)> | 16.1 | 3.6 |
| Completeness [%] | 94.5 | 94 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 2-2.5 M NACL, 0.1 M MES [PH 6-6.7], 10 MM DTT. |
