3ZI3
Crystal structure of the B24His-insulin - human analogue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 57.450, 57.450, 56.201 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.100 - 1.700 |
| R-factor | 0.17764 |
| Rwork | 0.175 |
| R-free | 0.22116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mso |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.545 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.120 | 0.670 |
| Number of reflections | 5435 | |
| <I/σ(I)> | 12.6 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.2 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3 | 0.075 M LI2SO4, PH 3.0 |
