3ZFS
Cryo-EM structure of the F420-reducing NiFe-hydrogenase from a methanogenic archaeon with bound substrate
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2 |
Detector | KODAK SO-163 FILM |
Number of Images | 80 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4 |
Software | Coot,UCSF Chimera,EMAN |
Symmetry | T |