3ZFS
Cryo-EM structure of the F420-reducing NiFe-hydrogenase from a methanogenic archaeon with bound substrate
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 80 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4 |
| Software | Coot,UCSF Chimera,EMAN |
| Symmetry | T |
