3ZEZ
Phage dUTPases control transfer of virulence genes by a proto- oncogenic G protein-like mechanism.(Staphylococcus bacteriophage 80alpha dUTPase with dUPNHPP).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 3 |
Unit cell lengths | 88.657, 88.657, 88.657 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.650 - 2.800 |
R-factor | 0.21999 |
Rwork | 0.216 |
R-free | 0.26362 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hqu |
RMSD bond length | 0.004 |
RMSD bond angle | 1.021 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.330 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.070 | 0.750 |
Number of reflections | 5949 | |
<I/σ(I)> | 23.9 | 4.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2-6% TERT-BUTANOL, 0.1M TRIS (PH 8.5), 30-50% MPD OR PEG400 |