3ZEU

Structure of a Salmonella typhimurium YgjD-YeaZ heterodimer bound to ATPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 1
Unit cell lengths	61.214, 68.084, 87.624
Unit cell angles	107.66, 92.63, 116.45

Refinement procedure

Resolution	29.085 - 1.653
R-factor	0.1604
Rwork	0.159
R-free	0.18630
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.147
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	RESOLVE
Refinement software	PHENIX ((PHENIX.REFINE: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.070	0.470
Number of reflections	135840
<I/σ(I)>	31.2	2.5
Completeness [%]	96.4	87.3
Redundancy	6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		16% PEG3350, 0.1M TRIS PH8.0, 0.2M AMMONIUM SULPHATE.