3ZEU
Structure of a Salmonella typhimurium YgjD-YeaZ heterodimer bound to ATPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.214, 68.084, 87.624 |
| Unit cell angles | 107.66, 92.63, 116.45 |
Refinement procedure
| Resolution | 29.085 - 1.653 |
| R-factor | 0.1604 |
| Rwork | 0.159 |
| R-free | 0.18630 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.470 |
| Number of reflections | 135840 | |
| <I/σ(I)> | 31.2 | 2.5 |
| Completeness [%] | 96.4 | 87.3 |
| Redundancy | 6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 16% PEG3350, 0.1M TRIS PH8.0, 0.2M AMMONIUM SULPHATE. |
