3ZE3
Crystal structure of the integral membrane diacylglycerol kinase - delta7
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.03320, 0.97944 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 75.060, 91.540, 143.650 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 51.894 - 2.050 |
| R因子 | 0.1969 |
| Rwork | 0.196 |
| R-free | 0.21670 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.003 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.536 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX (CDE) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 58.040 | 2.160 |
| 分解能 [Å] (高) | 2.050 | 2.050 |
| Rmerge_l_obs | 0.110 | 0.850 |
| 独立反射数 | 62716 | |
| <I/σ(I)> | 12.4 | 2 |
| 完全性 [%] | 99.7 | 99.5 |
| 冗長性 | 9.3 | 6.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | 277 | 3-5 %(V/V) 2-METHYL-2, 4-PENTANEDIOL, 0.1 M SODIUM CHLORIDE, 0.06 M MAGNESIUM ACETATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE) METHOD AT 4 DEGREE CELSIUS WITH THE 7.8 MONOACYLGLYCEROL (7.8 MAG) AS THE HOSTING LIPID. |
