3ZDW
Substrate and dioxygen binding to the endospore coat laccase CotA from Bacillus subtilis
Replaces: 1UVWExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14 |
Synchrotron site | ESRF |
Beamline | ID14 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 102.140, 102.140, 136.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.060 - 2.400 |
R-factor | 0.17942 |
Rwork | 0.178 |
R-free | 0.21058 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gsk |
RMSD bond length | 0.020 |
RMSD bond angle | 1.994 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.040 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.040 |
Number of reflections | 32860 |
<I/σ(I)> | 12.48 |
Completeness [%] | 99.9 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |