3ZDW
Substrate and dioxygen binding to the endospore coat laccase CotA from Bacillus subtilis
Replaces: 1UVWExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14 |
| Synchrotron site | ESRF |
| Beamline | ID14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 102.140, 102.140, 136.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.060 - 2.400 |
| R-factor | 0.17942 |
| Rwork | 0.178 |
| R-free | 0.21058 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gsk |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.994 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.040 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.040 |
| Number of reflections | 32860 |
| <I/σ(I)> | 12.48 |
| Completeness [%] | 99.9 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
