3ZDB
Structure of E. coli ExoIX in complex with the palindromic 5ov4 DNA oligonucleotide, di-magnesium and potassium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-04 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.238, 152.302, 34.522 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.670 - 1.470 |
| R-factor | 0.20425 |
| Rwork | 0.203 |
| R-free | 0.23054 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EXOIX FLAP1 FRAGMENT COMPLEX STRUCTURE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.440 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.670 | 1.550 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.050 | 0.240 |
| Number of reflections | 60541 | |
| <I/σ(I)> | 15.2 | 4.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M MAGNESIUM ACETATE, 15% (W/V) PEG-3350, pH 6.5 |
