3ZDA
Structure of E. coli ExoIX in complex with a fragment of the Flap1 DNA oligonucleotide, potassium and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.203, 154.820, 34.473 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.640 - 1.500 |
| R-factor | 0.21184 |
| Rwork | 0.211 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zd9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.547 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.640 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.100 | 0.480 |
| Number of reflections | 57905 | |
| <I/σ(I)> | 22.5 | 3.4 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 9.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.2 M MAGNESIUM ACETATE, 15% (W/V) PEG-3350, pH 6.0 |
