3YGS
APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 92.500, 92.500, 136.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.224 * |
Rwork | 0.224 |
R-free | 0.29900 |
Structure solution method | MIR |
RMSD bond length | 0.009 |
RMSD bond angle | 1.447 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.056 | 0.177 |
Total number of observations | 134080 * | |
Number of reflections | 12452 * | |
Completeness [%] | 98.6 | 93.2 |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 4 * | pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | HEPES | 100 (mM) | |
2 | 1 | reservoir | potassium sodium dihydrogen phosphate | 1.75 (M) | |
3 | 1 | reservoir | dithiothreitol | 10 (mM) |