3X43
Crystal structure of O-ureido-L-serine synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-07 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.480, 154.130, 118.520 |
Unit cell angles | 90.00, 90.48, 90.00 |
Refinement procedure
Resolution | 29.830 - 2.250 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q3d |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.090 | 0.475 |
Number of reflections | 122927 | |
<I/σ(I)> | 9.1 | 2.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | Tris-HCl, polyethylene glycol 8,000, KH2PO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |