3X42
Crystal structure of copper amine oxidase from Arthrobacter globiformis in the presence of sodium bromide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-09 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.684, 63.508, 158.099 |
| Unit cell angles | 90.00, 117.55, 90.00 |
Refinement procedure
| Resolution | 24.738 - 1.875 |
| R-factor | 0.1595 |
| Rwork | 0.158 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iu7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.083 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.960 |
| High resolution limit [Å] | 1.875 | 1.890 |
| Rmerge | 0.076 | 0.471 |
| Number of reflections | 267101 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.3 | 98.7 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |
