3X3Z
Copper amine oxidase from Arthrobacter globiformis: Aminoresorcinol form produced by anaerobic reduction with ethylamine hydrochloride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.548, 62.729, 157.647 |
Unit cell angles | 90.00, 117.62, 90.00 |
Refinement procedure
Resolution | 22.281 - 1.510 |
R-factor | 0.1615 |
Rwork | 0.161 |
R-free | 0.17830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.153 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.519 | 38.519 | 1.590 |
High resolution limit [Å] | 1.500 | 4.780 | 1.510 |
Rmerge | 0.092 | 0.056 | 0.404 |
Rpim | 0.024 | 0.163 | |
Total number of observations | 54557 | 260450 | |
Number of reflections | 260515 | ||
<I/σ(I)> | 17.3 | 39.8 | 4 |
Completeness [%] | 99.8 | 96.8 | 99.8 |
Redundancy | 7.1 | 6.6 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.8 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |