3X2V
Michaelis-like complex of cAMP-dependent Protein Kinase Catalytic Subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.310, 78.420, 79.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.170 - 1.770 |
R-factor | 0.1855 |
Rwork | 0.183 |
R-free | 0.22960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o22 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.252 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.172 | 1.820 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 38008 | |
Completeness [%] | 99.8 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 100mM MES pH 6.5, 5mM DTT, 15-20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |