3X2V

Michaelis-like complex of cAMP-dependent Protein Kinase Catalytic Subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	61.310, 78.420, 79.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.170 - 1.770
R-factor	0.1855
Rwork	0.183
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o22
RMSD bond length	0.010
RMSD bond angle	1.252
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.172	1.820
High resolution limit [Å]	1.770	1.770
Number of reflections	38008
Completeness [%]	99.8	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	100mM MES pH 6.5, 5mM DTT, 15-20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K