3X1H
hPPARgamma Ligand binding domain in complex with 5-oxo-tricosahexaenoic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.330, 61.960, 118.790 |
| Unit cell angles | 90.00, 102.86, 90.00 |
Refinement procedure
| Resolution | 29.930 - 2.300 |
| R-factor | 0.22981 |
| Rwork | 0.228 |
| R-free | 0.26636 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.763 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.180 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 26806 |
| Completeness [%] | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris-HCl PH 8, 0.7M sodium citrate, 1MM TCEP, 0.5MM EDTA, UNDER NITROGEN, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
